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Combining the data from this and the previous study and excluding inadequate specimens, the worldwide HPV prevalence in cervical carcinomas is 99.7 per cent.
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Only two of the 34 adequate samples were HPV-negative on all PCR tests, as against 13 of the 21 that usabio were usabio inadequate ( p< 0.001). Histological analysis of the 55 specimens revealed that 21 were qualitatively inadequate. Two (4 per cent) additional cases were only HPV DNA-positive by E1 and/or L1 consensus PCR. Type-specific E7 PCR for 14 high-risk HPV types detected HPV DNA in 38 (69 per cent) of the 55 originally HPV-negative and amplifiable specimens. No significant difference usabio was found in serology for HPV 16 proteins between the cases that were usabio originally HPV PCR-negative and -positive.
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Moreover, 55 of the 66 formerly HPV-negative biopsies were also reanalyzed by a sandwich procedure in which the outer sections in a series of sections are used for histological review, while the inner sections are assayed by three different HPV PCR assays targeting different open reading frames (ORFs). Serology for HPV 16 VLPs, E6, and E7 antibodies was performed on 49 of the 66 cases which were HPV-negative and a sample of 48 of the 866 cases which were HPV-positive in the original study. The formerly HPV-negative cases from this study have therefore been reanalyzed for usabio HPV serum antibodies and HPV DNA.
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A recent report that 93 per cent of invasive cervical cancers worldwide contain human papillomavirus (HPV) may be an underestimate, due to sample usabio inadequacy or integration events affecting the HPV L1 gene, which is the target of the polymerase chain reaction (PCR)-based test which was used.